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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Cocais; Embrapa Roraima. |
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Data corrente: |
08/11/2001 |
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Data da última atualização: |
06/06/2019 |
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Autoria: |
MIRANDA, I. P. de A.; RABELO, A.; BUENO, C. R.; BARBOSA, E. M.; RIBEIRO, M. N. S. |
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Afiliação: |
INPA, Manaus, AM. |
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Título: |
Frutos de palmeiras da Amazônia. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Manaus: INPA, 2001. |
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Páginas: |
118 p. |
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ISBN: |
85-211-0014-0 |
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Idioma: |
Português |
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Conteúdo: |
Acrocomia aculeata. Astrocaryum acaule Martius. Astrocaryum aculeatum. Astrocaryum gynacanthum Martius. Attalea attaleoides. Attalea monosperma. Attalea phalerata. Bactris bidentula. Bactris brogniarti. Bactris conccina. Bactris cuspidata. Barcella odora. Desmoncus polyacanthos. Elaeis guineensis. Elaeis oleifera. Euterpe catinga. Euterpe oleracea. Geonoma maxima var. maxima Martius. Iriartella setigera. Leopoldinia pulchra. Manicaria saccifera. Mauritia carana. Maximiliana maripa. Oenocarpus bacaba. |
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Palavras-Chave: |
Açaizinho; Agronomic characteres; Anaja; Bacabinha; Brasil; Bucu; Buritirana; Carana; Coqueiro javari; Curua; Frutos; Maraja; Maraja assu; Marfim vegetal; Mumbaca; Pachiubinha; Palha preta; Palm; Palmácea; Palmeira; Paxiuba; Piririma; Plant oils; Planting stock; Preservacao; Preservation; Pupunha do mato; Species; Tropical fruits; Tucuma; Ubim; Urucuri. |
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Thesagro: |
Açaí; Babaçu; Bacaba; Bacuri; Biodiversidade; Botânica; Buriti; Características Agronômicas; Comercialização; Dendê; Ecologia; Espécie; Fruta Tropical; Muda; Murumuru; Óleo Vegetal; Palmae; Palmeira Oleaginosa; Patauá; Tucum. |
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Thesaurus Nal: |
Amazonia; biodiversity; botany; Brazil; ecology; trade. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02462nam a2200877 a 4500
001 1346671
005 2019-06-06
008 2001 bl uuuu 00u1 u #d
020 $a85-211-0014-0
100 1 $aMIRANDA, I. P. de A.
245 $aFrutos de palmeiras da Amazônia.
260 $aManaus: INPA$c2001
300 $a118 p.
520 $aAcrocomia aculeata. Astrocaryum acaule Martius. Astrocaryum aculeatum. Astrocaryum gynacanthum Martius. Attalea attaleoides. Attalea monosperma. Attalea phalerata. Bactris bidentula. Bactris brogniarti. Bactris conccina. Bactris cuspidata. Barcella odora. Desmoncus polyacanthos. Elaeis guineensis. Elaeis oleifera. Euterpe catinga. Euterpe oleracea. Geonoma maxima var. maxima Martius. Iriartella setigera. Leopoldinia pulchra. Manicaria saccifera. Mauritia carana. Maximiliana maripa. Oenocarpus bacaba.
650 $aAmazonia
650 $abiodiversity
650 $abotany
650 $aBrazil
650 $aecology
650 $atrade
650 $aAçaí
650 $aBabaçu
650 $aBacaba
650 $aBacuri
650 $aBiodiversidade
650 $aBotânica
650 $aBuriti
650 $aCaracterísticas Agronômicas
650 $aComercialização
650 $aDendê
650 $aEcologia
650 $aEspécie
650 $aFruta Tropical
650 $aMuda
650 $aMurumuru
650 $aÓleo Vegetal
650 $aPalmae
650 $aPalmeira Oleaginosa
650 $aPatauá
650 $aTucum
653 $aAçaizinho
653 $aAgronomic characteres
653 $aAnaja
653 $aBacabinha
653 $aBrasil
653 $aBucu
653 $aBuritirana
653 $aCarana
653 $aCoqueiro javari
653 $aCurua
653 $aFrutos
653 $aMaraja
653 $aMaraja assu
653 $aMarfim vegetal
653 $aMumbaca
653 $aPachiubinha
653 $aPalha preta
653 $aPalm
653 $aPalmácea
653 $aPalmeira
653 $aPaxiuba
653 $aPiririma
653 $aPlant oils
653 $aPlanting stock
653 $aPreservacao
653 $aPreservation
653 $aPupunha do mato
653 $aSpecies
653 $aTropical fruits
653 $aTucuma
653 $aUbim
653 $aUrucuri
700 1 $aRABELO, A.
700 1 $aBUENO, C. R.
700 1 $aBARBOSA, E. M.
700 1 $aRIBEIRO, M. N. S.
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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Biblioteca |
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Origem |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/04/2023 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VASQUEZ, D. D. N.; PINHEIRO, D. H.; TEIXEIRA, L. A.; MOREIRA-PINTO, C. E.; MACEDO, L. L. P. de; SALLES-FILHO, A. L. O.; SILVA, M. C. M. da; LOURENCO, I. T.; MORGANTE, C. V.; SILVA, L. P. da; SA, M. F. G. de. |
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Afiliação: |
DANIEL D. N. VASQUEZ, Catholic University of Brasília; DANIELE H. PINHEIRO; LAYS A. TEIXEIRA, Catholic University of Brasília; CLIDIA E. MOREIRA-PINTO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ALVARO L. O. SALLES-FILHO, Federal University of Paraná; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; LUCIANO PAULINO DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Simultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Frontiers in Molecular Biosciences, v. 10, 2023. |
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DOI: |
https://doi.org/10.3389/fmolb.2023.1073721 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa. |
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Conteúdo: |
The cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that MenosThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the inj... Mostrar Tudo |
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Palavras-Chave: |
Bicudo do algodoeiro; Juvenile diol kinase; Juvenile hormone epoxide hydrolase; Polyethylenimine; Pragas do algodoeiro. |
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Thesagro: |
Algodão; Anthonomus Grandis; Inseto; Praga. |
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Thesaurus NAL: |
Chitosan; Cotton; Insect control; Insect pests. |
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Categoria do assunto: |
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O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153217/1/fmolb-10-1073721.pdf
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Marc: |
LEADER 03612naa a2200421 a 4500
001 2153271
005 2023-05-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fmolb.2023.1073721$2DOI
100 1 $aVASQUEZ, D. D. N.
245 $aSimultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil.$h[electronic resource]
260 $c2023
500 $aNa publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa.
520 $aThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that
650 $aChitosan
650 $aCotton
650 $aInsect control
650 $aInsect pests
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aInseto
650 $aPraga
653 $aBicudo do algodoeiro
653 $aJuvenile diol kinase
653 $aJuvenile hormone epoxide hydrolase
653 $aPolyethylenimine
653 $aPragas do algodoeiro
700 1 $aPINHEIRO, D. H.
700 1 $aTEIXEIRA, L. A.
700 1 $aMOREIRA-PINTO, C. E.
700 1 $aMACEDO, L. L. P. de
700 1 $aSALLES-FILHO, A. L. O.
700 1 $aSILVA, M. C. M. da
700 1 $aLOURENCO, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSILVA, L. P. da
700 1 $aSA, M. F. G. de
773 $tFrontiers in Molecular Biosciences$gv. 10, 2023.
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Embrapa Semiárido (CPATSA) |
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